Human CCL23 ELISA Kit (ab216169)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.54 pg/ml
- Range: 7.03 pg/ml - 450 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CCL23 ELISA Kit
CCL23 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 8.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 5.5% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.54 pg/ml -
検出範囲
7.03 pg/ml - 450 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 91 89% - 92% Serum 106 98% - 117% Other biological fluids 93 88% - 96% Hep Plasma 98 88% - 106% EDTA Plasma 101 98% - 106% Cit plasma 105 93% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human CCL23 ELISA Kit (ab216169) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL23 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL23 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
CCL23 (Macrophage inflammatory protein 3) is a chemokine involved in the recruitment of monocytes, neutrophils and resting T cells. CCL23 functions by inhibition myeloid progenitor cell proliferation. CCL23 is found at high levels in the synovial fluid of patients with rheumatoid arthritis.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CCL23 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CCL23 Capture Antibody (Lyophilized) 1 vial Human CCL23 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
組織特異性
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C C motif chemokine ligand 23
- C-C motif chemokine 23
- C6 beta chemokine
see all -
参照データベース
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
関連製品
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Alternative Versions
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ELISA kits
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 698 pg/mL in serum, 621 pg/mL in plasma (citrate), 689 pg/mL in plasma (heparin) and 592 pg/mL in plasma (EDTA).
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The concentrations of MIP3 were measured in duplicates, interpolated from the MIP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: synovial fluid 25% and THP-1 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 182 pg/mL in synovial fluid and 130 pg/mL in THP-1 supernatant.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP3 concentration was determined to be 797 pg/mL with a range of 229 – 1,754 pg/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab216169 は論文での使用が確認できていません。