Rat IL-17A ELISA Kit (ab214028)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IL-17A ELISA Kit
IL-17A キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3 3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 5 7% -
サンプルの種類
Cell culture supernatant, Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.1 pg/ml -
検出範囲
6.25 pg/ml - 400 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 113 105% - 117% Cell culture media 101 96% - 110% Cit plasma 110 108% - 112% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat -
製品の概要
Rat IL-17A ELISA Kit (ab214028) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-17A with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
INTERFERENCE
Recombinant mouse IL-17F was prepared at 50 ng/mL and 1 ng/mL and tested for interference. No interference with was observed.
SPECIES REACTIVITY
This kit recognizes rat IL-17A protein. It also reacts with mouse IL-17A protein.
Mouse and human species reactivity was determined by measuring 2-fold dilutions of mouse and human recombinant IL-17A protein (see below). Other species reactivity was not determined.
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特記事項
IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat IL-17a Capture Antibody 1 x 600µl 10X Rat IL-17a Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Rat IL-17a Lyophilized Recombinant Protein (ab78597) 2 vials Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
組織特異性
Restricted to activated memory T-cells. -
配列類似性
Belongs to the IL-17 family. -
翻訳後修飾
Found both in glycosylated and nonglycosylated forms. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CTLA 8
- CTLA-8
- CTLA8
see all -
参照データベース
- Entrez Gene: 301289 Rat
- SwissProt: Q61453 Rat
画像
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Background-subtracted data values (mean +/- SD) are graphed.
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The IL-17A standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed
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Rat spleen cells were cultured in the absence or presence of 5 µg/mL Concanavalin A for 48 hours. The concentrations of Il-17a were measured in neat supernatant samples in duplicates and interpolated from the IL-17a standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL‑17a concentration was determined to be 33.2 pg/mL in unstimulated, 187 pg/mL in Concanavalin A stimulated supernatants and undetectable in media (not shown).
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The concentrations of IL-17a were measured in duplicates, interpolated from the IL-17a standard curves and corrected for sample dilution. Undiluted samples are as follows: rat spleen cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17a concentration was determined to be 190 pg/mL in Concanavalin A treated rat spleen cell culture supernatant.
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Background-subtracted data values (n = 1) are graphed. Note that this kit is specific for IL-17a homodimer, it does not recognize IL-17f homodimer or IL-17a/f heterodimer.
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Two fold serial dilutions of rat, mouse and human recombinant IL-17a proteins were measured with this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed. O.D. values for human IL-17a protein were below background values. Note this kit reacts with mouse recombinant IL-17a protein. Note this kit does not react with human recombinant IL-17a protein.
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Background-subtracted data values (mean +/- SD) are graphed
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The IL-17A standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-17A was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 75BS.
100% pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-17A was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab214028 は 7 報の論文で使用されています。
- Zhou Y et al. Effects and early diagnostic value of lncRNA H19 on sepsis-induced acute lung injury. Exp Ther Med 23:279 (2022). PubMed: 35317444
- Wang H et al. Inhibiting PDGF-D alleviates the symptoms of HELLP by suppressing NF-κB activation. J Mol Endocrinol 66:233-243 (2021). PubMed: 33640869
- Jin Z et al. Protective effect of Qingre Huoxue decoction against myocardial infarction via PI3K/Akt autophagy pathway based on UPLC-MS, network pharmacology, and in vivo evidence. Pharm Biol 59:1607-1618 (2021). PubMed: 34818128
- Tang Z et al. Tryptophan promoted β-defensin-2 expression via the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine. RSC Adv 10:3371-3379 (2020). PubMed: 35497743
- Yu X & Xie Y Effect of dexmedetomidine combined with etomidate on IL-17A and S-100ß expression levels in rats with postoperative cognitive dysfunction. Exp Ther Med 20:275 (2020). PubMed: 33200000
- Hu X et al. RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll-like receptor signaling pathway. J Cell Physiol N/A:N/A (2019). PubMed: 31012109
- Feng F et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). ELISA ; Rat . PubMed: 30546393