Mouse VEGF R3 ELISA Kit (FLT4) (ab203367)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.97 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse VEGF R3 ELISA Kit (FLT4) -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8 4.59% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 7.14% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
17.97 pg/ml -
検出範囲
46.9 pg/ml - 3000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 85 81% - 88% Cell culture media 87 87% - 88% Hep Plasma 99 95% - 101% EDTA Plasma 100 96% - 104% Cit plasma 103 101% - 107% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Cow, Pig -
製品の概要
Mouse VEGF R3 ELISA Kit (FLT4) (ab203367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGF R3 (FLT4) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGF R3 (FLT4) with 17.97 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
The VEGF receptor family includes three members VEGF R1, VEGF R2, and VEGF R3 (FLT4). These receptors have a characteristic structure that includes 7 Ig-like domains in the extracellular domain and a cytoplasmic tyrosine kinase domain with a long kinase insert region. VEGF R3 is a tyrosine protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD. It plays an essential role in adult lymphangiogenesis, and in the development of the vascular network and cardiovascular system during embryonic development. VEGF R3 promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated VEGF R3 leads to enhanced production of VEGFC and VEGFA, creating a positive feedback loop that enhances FLT4 signaling.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse VEGF R3 Capture Antibody 1 x 600µl 10X Mouse VEGF R3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP2 1 x 6ml Mouse VEGF R3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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関連性
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells. -
細胞内局在
Cell membrane; Single-pass type I membrane protein. -
別名
- FLT 4
- FLT4
- FLT41
see all -
参照データベース
- Entrez Gene: 14257 Mouse
- SwissProt: P35917 Mouse
関連製品
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Alternative Versions
画像
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Background-subtracted data values (mean +/- SD) are graphed.
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The 1X dilution is 5% for mouse serum, and 2.5% for each of the plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.
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The 1X dilution is 50.0% for the lung supernatants, and 25.0% for the liver supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab203367 は論文での使用が確認できていません。