Recombinant human S100 beta protein (ab200489)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human S100 beta protein
S100 beta タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to bind human AGER in a functional ELISA.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKE QEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE -
予測される分子量
12 kDa including tags -
領域
1 to 92 -
タグ
His tag N-Terminus -
配列の追加情報
NP_006263 Includes a polyhistidine tag at the N-terminus, and has a calculated MW of 11.5 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 11 kDa in SDS-PAGE .
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab200489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris buffer, 0.87% Sodium chloride, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- NEF
- Protein S100 B
- Protein S100-B
see all -
機能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
組織特異性
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
配列類似性
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab200489 は論文での使用が確認できていません。