Anti-PROX1 抗体 [EPR19273] (ab199359)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19273] to PROX1
- Suitable for: ICC/IF, WB, IHC-P, ChIC/CUT&RUN-seq
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-PROX1 antibody [EPR19273]
PROX1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19273] to PROX1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, ChIC/CUT&RUN-seqmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human brain and fetal liver lysates; SH-SY5Y and HepG2 whole cell lysates; Mouse and rat brain and hippocampus lysates. IHC-P: Mouse and rat hippocampus tissues. ICC/IF: SH-SY5Y cells. ChIC/CUT&RUN-Seq: HepG2 cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19273 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199359の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/500.
|
|
WB |
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC is recommended for rat and mouse only |
|
ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5 µg |
特記事項 |
---|
ICC/IF
1/500. |
WB
1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for rat and mouse only |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5 µg |
ターゲット情報
-
機能
Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. -
組織特異性
Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the Prospero homeobox family.
Contains 1 Prospero-type homeobox DNA-binding domain. -
ドメイン
The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity. -
細胞内局在
Nucleus. RORG promotes its nuclear localization. - Information by UniProt
-
参照データベース
- Entrez Gene: 5629 Human
- Entrez Gene: 19130 Mouse
- Entrez Gene: 305066 Rat
- Omim: 601546 Human
- SwissProt: Q92786 Human
- SwissProt: P48437 Mouse
- Unigene: 741808 Human
- Unigene: 744931 Human
see all -
製品の状態
Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter. -
別名
- Homeobox prospero like protein antibody
- Homeobox prospero like protein PROX1 antibody
- Homeobox prospero-like protein PROX1 antibody
see all
画像
-
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 HepG2 (Human liver hepatocellular carcinoma cell line) cells and 5 µg of ab199359 [EPR19273]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
-
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling PROX1 with ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on neurons of mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
All lanes : Anti-PROX1 antibody [EPR19273] (ab199359) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-PROX1 antibody [EPR19273] (ab199359) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
HEK-293 doesn't express PROX1. PMID:18948102.
-
All lanes : Anti-PROX1 antibody [EPR19273] (ab199359) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : Rat hippocampus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time : Lane 1,2 and 3: minutes; Lane 4: 5 seconds.
-
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling PROX1 with ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on neurons of rat hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) and HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling PROX1 with ab199359 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on SH-SY5Y cell line.
Negative expression in HEK-293 cells. HEK-293 doesn't express PROX1. PMID:18948102.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab199359 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (16)
ab199359 は 16 報の論文で使用されています。
- Iwasa K et al. GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells. Neurochem Res 48:996-1008 (2023). PubMed: 36436172
- Hori Y et al. PIK3CA mutation correlates with mTOR pathway expression but not clinical and pathological features in Fibfibroipose vascular anomaly (FAVA). Diagn Pathol 17:19 (2022). PubMed: 35094709
- Nagahara T et al. Evaluation of the degree and distribution of lymphangiectasia in full-thickness canine small intestinal specimens diagnosed with lymphoplasmacytic enteritis and granulomatous lymphangitis. J Vet Med Sci 84:566-573 (2022). PubMed: 35283405
- Li Y et al. RAF1 promotes lymphatic metastasis of hypopharyngeal carcinoma via regulating LAGE1: an experimental research. J Transl Med 20:255 (2022). PubMed: 35668458
- Pal S et al. Nuclear localization of histamine receptor 2 in primary human lymphatic endothelial cells. Biol Open 11:N/A (2022). PubMed: 35776777