Extracellular Oxygen Consumption Reagent (ab197242)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Purified mitochondria, Suspension cells, Tissue
製品の概要
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製品名
Extracellular Oxygen Consumption Reagent
Extracellular Oxygen Consumption キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Tissue, Adherent cells, Suspension cells, Purified mitochondria -
アッセイタイプ
Cell-based (quantitative) -
製品の概要
Extracellular Oxygen Consumption Reagent ab197242 is an oxygen-sensitive fluorescent dye; its fluorescence is quenched by oxygen. The dye is used in a mix-and-read, 96-well fluorescence plate reader assay for the real-time kinetic analysis of extracellular oxygen consumption rates (OCR). The oxygen consumption rate is a measure of the cellular respiration rate, and of mitochondrial function.
The Extracellular Oxygen Consumption Reagent dye is used with an oil layer which is added on top of the assay medium to limit diffusion of oxygen into the assay medium. As mitochondrial respiration depletes the oxygen within the assay medium, quenching of the fluorescent dye is reduced, and the fluorescence signal increases proportionately.
The dye and a suitable oil are available together in Extracellular Oxygen Consumption Assay Kit ab197243.
The extracellular oxygen consumption assay that the dye is used in, is optimized for isolated mitochondria and cell cultures, and can be used with tissues, enzyme preparations, and small organisms.
The dye excites at 360-380 nm (max 380) and emits at 630-680 nm (max 650).
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特記事項
Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.
Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 96 tests 4 x 96 tests Extracellular O2 Consumption Reagent 1 vial 4 vials -
研究分野
関連製品
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Related Products
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画像
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Typical lifetime profile of Extracellular O2 Consumption Assay for adherent cells, treated with different ETC compounds, including Antimycin A (recommended as a Negative Control). The effect of Glucose Oxidase as a positive Signal Control is illustrated schematically.
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Excitation and emission spectra of Extracellular O2 Consumption Reagent. Left panel shows normalized excitation (Ex = 360-400nm; Peak 380nm). Right panel shows emission (Em = 630 - 680nm; Peak 650nm) in oxygenated and deoxygenated conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab197242 は 6 報の論文で使用されています。
- Zhu Y et al. miR-340-5p Mediates Cardiomyocyte Oxidative Stress in Diabetes-Induced Cardiac Dysfunction by Targeting Mcl-1. Oxid Med Cell Longev 2022:3182931 (2022). PubMed: 35126811
- Jiang N et al. Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion. Nat Commun 13:1511 (2022). PubMed: 35314680
- Sun J et al. Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway. Adipocyte 11:562-571 (2022). PubMed: 36053001
- Martí JM et al. Selective modulation by PARP-1 of HIF-1a-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions. Redox Biol 41:101885 (2021). PubMed: 33581682
- Zhu T et al. Ferroptosis Promotes Photodynamic Therapy: Supramolecular Photosensitizer-Inducer Nanodrug for Enhanced Cancer Treatment. Theranostics 9:3293-3307 (2019). PubMed: 31244955
- Song W et al. Activin signaling mediates muscle-to-adipose communication in a mitochondria dysfunction-associated obesity model. Proc Natl Acad Sci U S A N/A:N/A (2017). PubMed: 28739899