HRP Anti-alpha 1 Sodium Potassium ATPase 抗体 [464.6] (ab196696)
Key features and details
- HRP Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
alpha 1 Sodium Potassium ATPase 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [464.6] to alpha 1 Sodium Potassium ATPase -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human Brain, Mouse Brain and Rat Brain tissue lysates. IHC-P: Normal human kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
464.6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196695)
- Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free (ab237969)
- Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196696の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 112 kDa).
Abcam recommends using 5% BSA as the blocking agent. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 112 kDa). Abcam recommends using 5% BSA as the blocking agent. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻訳後修飾
Phosphorylation on Tyr-10 modulates pumping activity. -
細胞内局在
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 476 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 24211 Rat
- Omim: 182310 Human
- SwissProt: P05023 Human
- SwissProt: Q8VDN2 Mouse
- SwissProt: P06685 Rat
- Unigene: 371889 Human
see all -
別名
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
see all
画像
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IHC image of alpha 1 Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196696 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196696 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab196696 は論文での使用が確認できていません。