Anti-Maxi Potassium channel alpha/SLO 抗体 [L6/60] (ab192759)
Key features and details
- Mouse monoclonal [L6/60] to Maxi Potassium channel alpha/SLO
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Maxi Potassium channel alpha/SLO antibody [L6/60]
Maxi Potassium channel alpha/SLO 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [L6/60] to Maxi Potassium channel alpha/SLO -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Mouse and Rat Brain This antibody gave a positive result in IHC in the following FFPE tissue: Rat Normal Brain.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
L6/60 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192759の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 137 kDa).
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 137 kDa). |
ターゲット情報
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機能
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX). -
組織特異性
Widely expressed. Except in myocytes, it is almost ubiquitously expressed. -
関連疾患
Generalized epilepsy and paroxysmal dyskinesia -
配列類似性
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.
Contains 1 RCK N-terminal domain. -
ドメイン
The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.
The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+).
The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.
The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation. -
翻訳後修飾
Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.
Palmitoylation by ZDHHC22 and ZDHHC23 within the intracellular linker between the S0 and S1 transmembrane domains regulates localization to the plasma membrane. Depalmitoylated by LYPLA1 and LYPLAL1, leading to retard exit from the trans-Golgi network. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3778 Human
- Entrez Gene: 16531 Mouse
- Entrez Gene: 83731 Rat
- Omim: 600150 Human
- SwissProt: Q12791 Human
- SwissProt: Q08460 Mouse
- SwissProt: Q62976 Rat
- Unigene: 144795 Human
see all -
別名
- subfamily M subunit alpha-1 antibody
- BK channel antibody
- BKCA alpha antibody
see all
画像
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All lanes : Anti-Maxi Potassium channel alpha/SLO antibody [L6/60] (ab192759) at 5 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 137 kDa
Additional bands at: 110 kDa, 28 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
IHC image of Maxi Potassium channel alpha/SLO antibody [L6/60] staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab192759 は 4 報の論文で使用されています。
- Shen X et al. Alteration of sphingosine-1-phosphate with aging induces contractile dysfunction of colonic smooth muscle cells via Ca2+ -activated K+ channel (BKCa ) upregulation. Neurogastroenterol Motil 33:e14052 (2021). PubMed: 33452855
- Kshatri A et al. Differential regulation of BK channels by fragile X mental retardation protein. J Gen Physiol 152:N/A (2020). PubMed: 32275741
- Sun X et al. A lysosomal K+ channel regulates large particle phagocytosis by facilitating lysosome Ca2+ release. Sci Rep 10:1038 (2020). PubMed: 31974459
- Hartmann S et al. ß-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. J Neurosci 38:3480-3494 (2018). PubMed: 29507146