Rat IgG ELISA Kit (ab189578)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 55 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IgG ELISA Kit
IgG キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3 2.66% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 3.61% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
55 pg/ml -
検出範囲
0.31 ng/ml - 20 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Antibody Diluent 4B 103.7 99.4% - 110.97% OF DMEM Medium 76.5 62.8% - 94% 10F DMEM Medium 79.1 67.5% - 98% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat
非交差種: Goat, Cow, Pig -
製品の概要
Rat IgG ELISA Kit (ab189578) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IgG with 55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes native rat IgG in serum, plasma and cell culture supernatant samples.
SPECIES REACTIVITY
This kit recognizes rat IgG protein and cross reacts with hamster IgG.
Other species reactivity was determined by measuring 1 to 1 million diluted serum samples of various species, interpolating the protein concentrations from the rat IgG standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in rat serum assayed at the same dilution.
Reactivity < 0.3% was determined for the following species: Mouse, Guinea Pig, Rabbit, Dog, Goat, Pig, Cow, Human, Chicken
Hamster serum displays 71.4% cross-reactvity.
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特記事項
There are four classes of immunoglobulins in rat: IgA, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. In rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. Typically rat serum and plasma samples contain about 7 to 10 mg/ml of IgG.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Rat IgG Capture Antibody 1 x 600µl 10 x 600µl 1 x 600µl 10X Rat IgG Detector Antibody 1 x 600µl 10 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4B 1 x 6ml 10 x 6ml 1 x 6ml Plate Seals 1 unit 10 units 1 unit Rat IgG Lyophilized Purified Protein 2 vials 20 vials 2 vials Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 1 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究分野
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細胞内局在
Secreted -
別名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
関連製品
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ELISA kits
画像
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Example of rat IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:4,000,000 rat serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat IgG standard curve. The rat IgG standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Native rat IgG was measured in the following biological samples in a 2‑fold dilution series. Sample dilutions are made in Sample Diluent NS.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab189578 は 7 報の論文で使用されています。
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- Hata J et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151
- Chapy H & Kagan L Evaluation of the Effects of Animal Growth and Previous Exposure on the Pharmacokinetics of Rituximab in Rats. J Pharm Sci 107:1987-1994 (2018). Sandwich ELISA ; Rat . PubMed: 29551353
- Eom Y et al. The Effect of Ambient Titanium Dioxide Microparticle Exposure to the Ocular Surface on the Expression of Inflammatory Cytokines in the Eye and Cervical Lymph Nodes. Invest Ophthalmol Vis Sci 57:6580-6590 (2016). PubMed: 27918832
- Regal JF et al. Role of IgM and angiotensin II Type I receptor autoantibodies in local complement activation in placental ischemia-induced hypertension in the rat. Mol Immunol 78:38-47 (2016). PubMed: 27588825