HRP Anti-Sodium Potassium ATPase 抗体 [EP1845Y] - Plasma Membrane Loading Control (ab185065)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
Sodium Potassium ATPase 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EP1845Y] to Sodium Potassium ATPase - Plasma Membrane Loading Control -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Tilapia -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in human stomach carcinoma tissue lysate as well as the following whole cell lysates: HeLa; HEK293 IHC: Human brain (cerebral cortex), rat brain and mouse brain.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1845Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free (ab167390)
- Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab197713)
- Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab198366)
- Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (ab198367)
- PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab209299)
- Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab210143)
- Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab274883)
- APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab314288)
- Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185065の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
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WB |
1/5000. Predicted molecular weight: 113 kDa.
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特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
WB
1/5000. Predicted molecular weight: 113 kDa. |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻訳後修飾
Phosphorylation on Tyr-10 modulates pumping activity. -
細胞内局在
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Entrez Gene: 11928 Mouse
- Entrez Gene: 11931 Mouse
- Entrez Gene: 27222 Mouse
- Entrez Gene: 24211 Rat
- Entrez Gene: 25650 Rat
see all -
別名
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
画像
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All lanes : HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab185065) at 1/5000 dilution (Milk blocking 3%)
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185065 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded Human brain (cerebral cortex). The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab185065, 1µg/ml overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal mouse brain, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab185065, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset background control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of Sodium Potassium ATPase staining in a section of formalin-fixed paraffin-embedded normal rat brain, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab185065, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset background control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab185065 は 8 報の論文で使用されています。
- Saran C et al. Novel Bile Acid-Dependent Mechanisms of Hepatotoxicity Associated with Tyrosine Kinase Inhibitors. J Pharmacol Exp Ther 380:114-125 (2022). PubMed: 34794962
- Wang C et al. The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells. Anim Nutr 10:12-18 (2022). PubMed: 35601254
- He X et al. LYSMD3: A mammalian pattern recognition receptor for chitin. Cell Rep 36:109392 (2021). PubMed: 34289364
- Beaudoin JJ et al. Role of Organic Solute Transporter Alpha/Beta in Hepatotoxic Bile Acid Transport and Drug Interactions. Toxicol Sci N/A:N/A (2020). PubMed: 32294204
- Zhao S et al. High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugate. J Pathol Clin Res 5:12-24 (2019). PubMed: 30191677
- Choi EJ et al. Gambogic Acid and Its Analogs Inhibit Gap Junctional Intercellular Communication. Front Pharmacol 9:814 (2018). PubMed: 30104974
- Marino A et al. S1P receptor 1-Mediated Anti-Renin-Angiotensin System Cardioprotection: Pivotal Role of Mast Cell Aldehyde Dehydrogenase Type 2. J Pharmacol Exp Ther 362:230-242 (2017). PubMed: 28500264
- Burgess KA et al. Western blot analysis of cells encapsulated in self-assembling peptide hydrogels. Biotechniques 63:253-260 (2017). PubMed: 29235971