Anti-pro Caspase-1 + p10 + p12 抗体 [EPR16883] (ab179515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]
pro Caspase-1 + p10 + p12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12 -
由来種
Rabbit -
特異性
This antibody has low affinity in Ms/Rt brain, H1299 and A549
-
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Untreated THP-1 and treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysates; Rat lung and spleen lysates; Human fetal lung lysates; J774A.1 and RAW 264.7 whole cell lysates. Mouse and rat brain lysates, A549 cells, NCI-H1299 cells. IP: RAW 264.7 whole cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16883 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179515の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000. Detects a band of approximately 45, 42, 35, 12, 10 kDa (predicted molecular weight: 45, 42, 35, 12, 10 kDa).
|
IP |
1/100.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 45, 42, 35, 12, 10 kDa (predicted molecular weight: 45, 42, 35, 12, 10 kDa). |
IP
1/100. |
ターゲット情報
-
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 834 Human
- Entrez Gene: 12362 Mouse
- Entrez Gene: 25166 Rat
- Omim: 147678 Human
- SwissProt: P29466 Human
- SwissProt: P29452 Mouse
- SwissProt: P43527 Rat
-
別名
- CASP1 antibody
- Caspase 1 apoptosis related cysteine peptidase antibody
- ICE antibody
see all
画像
-
All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 6 : Mouse spleen lysate
Lane 7 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45, 42, 35, 12, 10 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: 7 seconds
ab179515 has low affinity in Ms/Rt brain,H1299 and A549
-
All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution
Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45, 42, 35, 12, 10 kDa
Observed band size: 10,12,35,42,45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20 and p10(p12) subunits. PMID: 8662843. The product recognizes p10(p12) cleavage fragment where the immunogen is located.
-
All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution
Lane 1 : Rat lung lysate
Lane 2 : Human fetal lung lysate
Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45, 42, 35, 12, 10 kDa
Observed band size: 42,45 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.
-
pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).
Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab179515 in RAW 264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
-
Different batches of ab179515 were tested on RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) lysate at 1.7 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 42,45 kDa.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (228)
ab179515 は 228 報の論文で使用されています。
- Li Y et al. Bacterial outer membrane vesicles-based therapeutic platform eradicates triple-negative breast tumor by combinational photodynamic/chemo-/immunotherapy. Bioact Mater 20:548-560 (2023). PubMed: 35846843
- Chen Y et al. Linear ubiquitination of LKB1 activates AMPK pathway to inhibit NLRP3 inflammasome response and reduce chondrocyte pyroptosis in osteoarthritis. J Orthop Translat 39:1-11 (2023). PubMed: 36514784
- Wu W et al. NLRP3 inflammasome activation in gestational diabetes mellitus placentas is associated with hydrogen sulfide synthetase deficiency. Exp Ther Med 23:94 (2022). PubMed: 34976136
- Chen B et al. The neuroprotective mechanism of lithium after ischaemic stroke. Commun Biol 5:105 (2022). PubMed: 35115638
- Ge C et al. Silencing of TLR4 Inhibits Atrial Fibrosis and Susceptibility to Atrial Fibrillation via Downregulation of NLRP3-TGF-β in Spontaneously Hypertensive Rats. Dis Markers 2022:2466150 (2022). PubMed: 35860690