Anti-ROX 抗体 [EPR11721] (ab173573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11721] to ROX
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ROX antibody [EPR11721]
ROX 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11721] to ROX -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A431, Raji, Jurkat and HeLa whole cell lysate (ab150035). A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11721 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. -
組織特異性
Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines. -
配列類似性
Belongs to the MINA53/NO66 family.
Contains 1 JmjC domain. -
細胞内局在
Nucleus. Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 540466 Cow
- Entrez Gene: 84864 Human
- Entrez Gene: 67014 Mouse
- Entrez Gene: 100174658 Orangutan
- Omim: 612049 Human
- SwissProt: Q5EA24 Cow
- SwissProt: Q8IUF8 Human
- SwissProt: Q8CD15 Mouse
see all -
別名
- 60S ribosomal protein L27a histidine hydroxylase antibody
- Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
- FLJ14393 antibody
see all
画像
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All lanes : Anti-ROX antibody [EPR11721] (ab173573) at 1/1000 dilution
Lane 1 : A431 cell lysates
Lane 2 : Raji cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
Immunofluorescence analysis of A431 cells labeling ROX with ab173573 at a 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab173573 は 1 報の論文で使用されています。
- Wang Q et al. MDIG promotes cisplatin resistance of lung adenocarcinoma by regulating ABC transporter expression via activation of the WNT/ß-catenin signaling pathway. Oncol Lett 18:4294-4307 (2019). PubMed: 31579066