Anti-nNOS (neuronal) (phospho S847) 抗体 (ab16650)
Key features and details
- Rabbit polyclonal to nNOS (neuronal) (phospho S847)
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-nNOS (neuronal) (phospho S847) antibody
nNOS (neuronal) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to nNOS (neuronal) (phospho S847) -
由来種
Rabbit -
特異性
This antibody shows a reduction in signal when blocked with unmodified nNOS (neuronal) peptide in WB, however when tested in ELISA, it showed less than 2% cross reactivity with the unmodified protein. -
アプリケーション
適用あり: IHC-FoFr, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Xenopus laevis, Zebrafish, Apteronotus leptorhynchus -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Mouse nNOS (neuronal), phosphorylated at S847.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16650の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr | (1) |
1/3000.
|
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
|
特記事項 |
---|
IHC-FoFr
1/3000. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
ターゲット情報
-
機能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. -
組織特異性
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Contains 1 PDZ (DHR) domain. -
ドメイン
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14. -
翻訳後修飾
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro). -
細胞内局在
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines. - Information by UniProt
-
参照データベース
- Entrez Gene: 4842 Human
- Entrez Gene: 18125 Mouse
- Entrez Gene: 100009243 Rabbit
- Entrez Gene: 24598 Rat
- Entrez Gene: 60658 Zebrafish
- Omim: 163731 Human
- SwissProt: P29475 Human
- SwissProt: Q9Z0J4 Mouse
see all -
別名
- 2310005C01Rik antibody
- BNOS antibody
- Constitutive NOS antibody
see all
画像
-
All lanes : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control)
Lane 4 : Forebrain (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 5 : Spinal Cord (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 7 : Forebrain (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lane 8 : Spinal Cord (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16650 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
All lanes : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
Lanes 1-3 : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 4 : nNOS antibody at 1/2500 dilution
Lanes 1 & 4 : mouse forebrain
Lane 2 : mouse forebrain with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981) at 1 µg/ml
Lane 3 : mouse forebrain with corresponding unmodified nNOS (neuronal) peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 160 kDa
Observed band size: 160 kDa -
Immunostaining using Rabbit polyclonal to nNOS (neuronal) (phospho S847) (ab16650) on rat brain tissue sections (30 micron free floating). ab16650 was used at a dilution of 1/3000 and incubated for 18 hours at RT (in PBS triton 0.3%). Secondary Antibody Goat anti-rabbit alexa Fluor 488 was used at a dilution of 1/1000. The image shows cytoplasmic staining of CNS neurons with ab16650 in naïve rats; the staining being observed in the soma and processes of these neurons. The staining was quenched by pre-incubation with peptide against phospho S847 (ab16981), but not by the control peptide (ab57047) indicating that ab16650 is specific for nNos phosphorylated at S847. Protocol: Rats were perfused-fixed with 4% paraformaldehyde. Tissues were post-fixed overnight in the same fixative and then cryoprotected in 20% sucrose overnight. Following embedding in OCT and freezing, tissues were cut and immunostained using the 'free floating’ technique.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab16650 は 15 報の論文で使用されています。
- Uda M et al. Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15:e0243660 (2020). PubMed: 33296434
- Alhaddad H et al. Effects of chronic ethanol consumption on the expression of GLT-1 and neuroplasticity-related proteins in the nucleus accumbens of alcohol-preferring rats. Brain Res Bull 165:272-280 (2020). PubMed: 33075417
- Sharma NM et al. Exercise training augments neuronal nitric oxide synthase dimerization in the paraventricular nucleus of rats with chronic heart failure. Nitric Oxide 87:73-82 (2019). PubMed: 30878404
- Smith ACW et al. Accumbens nNOS Interneurons Regulate Cocaine Relapse. J Neurosci 37:742-756 (2017). PubMed: 28123012
- Rice FL et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962