Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392)
Key features and details
- Sample type: Cell Lysate
製品の概要
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製品名
Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail -
サンプルの種類
Cell Lysate -
種交差性
交差種: Human
非交差種: Mouse, Rat -
製品の概要
This Epithelial-Mesenchymal Transition (EMT) western blot cocktail is designed to determine the relative abundance of canonical EMT markers. EMT is a process in which a cell transitions from an epithelial-like (attached, polarized) state to a mesenchymal-like (unattached, migratory) state and such transitions occur during development and metastasis. Underlying the morphological cell changes during EMT are transcription factor programs, biochemical cascades and alterations to the cytoskeleton. Beta-Catenin is a transcription factor in the Wnt signaling pathway and is involved in the regulation of cell adhesion; beta-Catenin is typically more abundant in epithelial-like cells. Vimentin is an intermediate filament typically found in non-epithelial and mesenchymal cells. Alpha Smooth Muscle Actin and GAPDH are also included in this cocktail as loading controls (Alpha Smooth Muscle Actin is reported to be more highly expressed in mesenchymal-like cells, but we find it to be widely expressed in transformed cell lines).
These four readouts are easily resolved by western blot given their different molecular weights. Because this western blot cocktail is a mix of rabbit and mouse monoclonal primary antibodies.
A secondary antibody cocktail of HRP conjugated antibodies (anti-mouse and anti-rabbit) is provided.
Expected and observed MWs:
- Beta-Catenin 92kDa
- Vimentin 54kDa
- Smooth Muscle Actin 42kDa
- GAPDH 36kDa
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アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 200 µl 2500X HRP Conjugated Secondary Antibody Cocktail 1 vial 250X EMT WB Cocktail 1 vial -
研究分野
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別名
- 38 kDa BFA-dependent ADP-ribosylation substrate
- a actin
- AAT6
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参照データベース
- Entrez Gene: 2597 Human
- Entrez Gene: 24383 Human
- Entrez Gene: 193060 Human
- Entrez Gene: 1499 Human
- Entrez Gene: 59 Human
- Omim: 138400 Human
- Omim: 7431 Human
- Omim: 116806 Human
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157392の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
Expected and observed MWs: Beta-Catenin 92kDa Vimentin 54kDa Smooth Muscle Actin 42kDa GAPDH 36kDa |
特記事項 |
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WB
Use at an assay dependent concentration. Expected and observed MWs: Beta-Catenin 92kDa Vimentin 54kDa Smooth Muscle Actin 42kDa GAPDH 36kDa |
画像
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Primary antibody: all lanes EMT WB cocktail (ab157392) diluted to 1X in 4% milk/PBS.
Lane 1. MDA-MB-231 cell lysate
Lane 2. SH SY5Y cell lysate
Lane 3. HeLa cell lysate
Lane 4. Jurkat cell lysate.
All lysates loaded 20 µg per lane. Lysates were generated in RIPA lysis buffer (ab156034) and briefly boiled in sample buffer before loading on SDS-PAGE.
Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS. -
MCF7 is epithelial-like and MDA-MB-231 cells are mesenchymal-like based on the relative expression of beta-Catenin and Vimentin between the cell lines.
Primary antibody: all lanes EMT WB cocktail diluted to 1X in 5% milk/PBS.
Lane 1. MCF7 cell lysate
Lane 2. MDA-MB-231 cell lysate
All lysates loaded 20 µg per lane. Lysates were generated in SDS lysis buffer and briefly boiled in sample buffer before loading on SDS-PAGE.
Secondary antibody: All lanes using the included HRP Conjugated Secondary Antibody Cocktail (included with ab157392) diluted to 1X in 5% milk/PBS.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab157392 は 2 報の論文で使用されています。
- Jarroux J et al. HOTAIR lncRNA promotes epithelial-mesenchymal transition by redistributing LSD1 at regulatory chromatin regions. EMBO Rep 22:e50193 (2021). PubMed: 33960111
- Matsushima H et al. Sox9 expression in carcinogenesis and its clinical significance in intrahepatic cholangiocarcinoma. Dig Liver Dis 47:1067-75 (2015). WB . PubMed: 26341967