Anti-Angiopoietin 2/ANG2 抗体 [EPR2891(2)] (ab155106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2891(2)] to Angiopoietin 2/ANG2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]
Angiopoietin 2/ANG2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2891(2)] to Angiopoietin 2/ANG2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Angiopoietin 2/ANG2. The exact sequence is proprietary.
Database link: O15123 -
ポジティブ・コントロール
- WB: Mouse small intestine tissue lysate - total protein (ab7939), TF1, HUVEC and ECV-304 cell lysates. Mouse spleen and rat kidney tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2891(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155106の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 285 Human
- Entrez Gene: 11601 Mouse
- Entrez Gene: 89805 Rat
- Omim: 601922 Human
- SwissProt: O15123 Human
- SwissProt: O35608 Mouse
- SwissProt: O35462 Rat
- Unigene: 583870 Human
see all -
別名
- AGPT 2 antibody
- Agpt2 antibody
- ANG 2 antibody
see all
画像
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/5000 dilution (purified)
Lane 1 : TF-1 whole cell lysate
Lane 2 : HUVEC whole cell lysate
Lane 3 : ECV-304 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (ab155106) at 1/1000 dilution (unpurified)
Lane 1 : TF1 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 57 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab155106 は 32 報の論文で使用されています。
- Kalfon T et al. Gastric Cancer-Derived Extracellular Vesicles (EVs) Promote Angiogenesis via Angiopoietin-2. Cancers (Basel) 14:N/A (2022). PubMed: 35740619
- Li J & Gao S HOXB5-activated ANGPT2 promotes the proliferation, migration, invasion and angiogenic effect of esophageal cancer cells via activating ERK/AKT signaling pathway. Exp Ther Med 24:585 (2022). PubMed: 35949323
- Zhu J et al. MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma. Cell Death Dis 13:727 (2022). PubMed: 35987690
- Sun X et al. TPTEP1 suppresses high glucose-induced dysfunction in retinal vascular endothelial cells by interacting with STAT3 and targeting VEGFA. Acta Diabetol 58:759-769 (2021). PubMed: 33576890
- Osawa Y et al. Blood angiopoietin-2 predicts liver angiogenesis and fibrosis in hepatitis C patients. BMC Gastroenterol 21:55 (2021). PubMed: 33557759