(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor (ab141122)
Key features and details
- Protein kinase C (PKC) inhibitor. Intermediate in lipid metabolism.
- CAS Number: 6865-14-1
- Purity: > 98%
- Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
(±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor -
製品の詳細
Protein kinase C (PKC) inhibitor. Intermediate in lipid metabolism. -
生理活性の詳細
Protein kinase C (PKC) inhibitor. Intermediate in long-chain fatty acid metabolism able to disrupt cell membranes.
Also available in simple stock solutions (ab146730) - add 1 ml of water to get an exact, ready-to-use concentration. -
精製度
> 98% -
CAS 番号
6865-14-1 -
構造式
製品の特性
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体系名
3-Carboxy-N,N,N-trimethyl-2-[(1-oxohexadecyl)oxy]-1-propanaminium chloride -
分子量
436.07 -
分子式
C23H46ClNO4 -
PubChem 登録番号
363417 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 10 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
[Cl-].O=C(CCCCCCCCCCCCCCC)OC(CC(=O)O)C[N+](C)(C)C -
由来
Synthetic
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研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab141122の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure of ab141122, (±)-Palmitoylcarnitine chloride, Protein kinase C (PKC) inhibitor
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ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab141122 は論文での使用が確認できていません。