Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (ab139474)
Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based -
全工程の試験時間
0h 30m -
種交差性
交差種: Mammals, Other species -
製品の概要
Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.
This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.
Review other dyes and kits for nucleolar staining, or the live cell staining fluorescent dyes guide
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試験プラットフォーム
Fluorescence microscope
製品の特性
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保存方法
Store at -80°C. Please refer to protocols. -
内容 500 tests 10X Assay Buffer 1 x 15ml Actinomycin D Control 1 x 125µg Green Detection Reagent 1 x 50µl Red Detection Reagent 1 x 50µl -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab139474 は 1 報の論文で使用されています。
- Ramamurthy B et al. Three-Dimensional Cellular Raman Analysis: Evidence of Highly Ordered Lipids Within Cell Nuclei. J Histochem Cytochem 66:889-902 (2018). PubMed: 30138043