Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (ab137994)
Key features and details
- Sensitivity: 1.6 pg/ml
- Range: 7.8 pg/ml - 125 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)
Thrombin-Antithrombin Complex キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.8% -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.6 pg/ml -
検出範囲
7.8 pg/ml - 125 pg/ml -
添加回収試験
97 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
TAT Complexes Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TAT Complexes levels in plasma and cell culture supernatants.
A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse TAT Complexes Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml TAT Complexes Microplate (12 x 8 well strips) 1 unit TAT Complexes Standard 1 vial -
研究分野
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関連性
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
細胞内局在
Secreted -
別名
- antithrombin III
- AT3
- AT3D
see all -
参照データベース
- Entrez Gene: 11905 Mouse
- Entrez Gene: 14061 Mouse
- SwissProt: P32261 Mouse
- SwissProt: P19221 Mouse
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab137994 は 16 報の論文で使用されています。
- Hultström M et al. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients. Biomedicines 10:N/A (2022). PubMed: 35740360
- Yuan C et al. Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling. Cell Death Dis 13:722 (2022). PubMed: 35982051
- Kaspi H et al. MSC-NTF (NurOwn®) exosomes: a novel therapeutic modality in the mouse LPS-induced ARDS model. Stem Cell Res Ther 12:72 (2021). PubMed: 33468250
- Cheng N et al. Targeting Ga13-integrin interaction ameliorates systemic inflammation. Nat Commun 12:3185 (2021). PubMed: 34045461
- Ghosh AK et al. Pharmacological inhibition of PAI-1 alleviates cardiopulmonary pathologies induced by exposure to air pollutants PM2.5. Environ Pollut 287:117283 (2021). PubMed: 34426376