Anti-VEGF Receptor 2 抗体 [EPRER16Y] (ab134191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRER16Y] to VEGF Receptor 2
- Suitable for: ELISA, WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VEGF Receptor 2 antibody [EPRER16Y]
VEGF Receptor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPRER16Y] to VEGF Receptor 2 -
由来種
Rabbit -
特異性
It is predicted that this antibody does not cross react with other members of the VEGF receptor family.
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アプリケーション
適用あり: ELISA, WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human VEGF Receptor 2 aa 1-800. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human placenta lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPRER16Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134191の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa).
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IP |
1/20.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 210, 230 kDa (predicted molecular weight: 151 kDa). |
IP
1/20. |
ターゲット情報
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機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
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別名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
画像
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Anti-VEGF Receptor 2 antibody [EPRER16Y] (ab134191) at 1/500 dilution + Human placenta lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 151 kDa
Observed band size: 210,230 kDa why is the actual band size different from the predicted? -
ELISA analysis of Hu VEGFR2 recombinant protein at 1000 ng/mL with ab134191. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab134191 は 21 報の論文で使用されています。
- Sun X & Liu B Donepezil ameliorates oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cell dysfunction via the SIRT1/FOXO3a/NF-κB pathways. Bioengineered 13:7760-7770 (2022). PubMed: 35286233
- Luo Z et al. LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis. Exp Ther Med 24:622 (2022). PubMed: 36160904
- Wu Q et al. KIF2A participates in the progression of hepatocellular carcinoma and angiogenesis by interacting with Notch1. Exp Ther Med 24:683 (2022). PubMed: 36277155
- Liang R et al. Dihydroartemisinin inhibits the tumorigenesis and invasion of gastric cancer by regulating STAT1/KDR/MMP9 and P53/BCL2L1/CASP3/7 pathways. Pathol Res Pract 218:153318 (2021). PubMed: 33370709
- Zhang P et al. Distinct mRNAs in Cancer Extracellular Vesicles Activate Angiogenesis and Alter Transcriptome of Vascular Endothelial Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33921957