Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) 抗体 [EPR2611Y] (ab134186)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y]
Phospholipase C gamma 1/PLC-gamma-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2611Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) -
由来種
Rabbit -
特異性
This antibody detects Phospholipase C gamma 1/PLC-gamma-1 phosphorylated on Tyrosine 783.
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アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783).
Database link: P19174 -
ポジティブ・コントロール
- WB: Jurkat cell lysate treated with Pervanadate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2611Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134186の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
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特記事項 |
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WB
1/5000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa). |
ターゲット情報
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機能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
配列類似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻訳後修飾
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
細胞内局在
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 5335 Human
- Omim: 172420 Human
- SwissProt: P19174 Human
- Unigene: 268177 Human
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別名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
画像
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All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody [EPR2611Y] (ab134186) at 1/10000 dilution
Lane 1 : Jurkat cell lysate untreated
Lane 2 : Jurkat cell lysate treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab134186 は 1 報の論文で使用されています。
- Xie L et al. FGFR2 gene amplification in gastric cancer predicts sensitivity to the selective FGFR inhibitor AZD4547. Clin Cancer Res 19:2572-83 (2013). PubMed: 23493349