Anti-ESD 抗体 [EPR8447] (ab133631)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8447] to ESD
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ESD antibody [EPR8447]
ESD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8447] to ESD -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human ESD aa 250-350 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HEK293T, K562, Jurkat, Caco 2 and HeLa cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8447 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133631の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
Serine hydrolase involved in the detoxification of formaldehyde. -
配列類似性
Belongs to the esterase D family. -
細胞内局在
Cytoplasm. Cytoplasmic vesicle. - Information by UniProt
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参照データベース
- Entrez Gene: 2098 Human
- Entrez Gene: 13885 Mouse
- Entrez Gene: 290401 Rat
- Omim: 133280 Human
- SwissProt: P10768 Human
- SwissProt: Q9R0P3 Mouse
- SwissProt: B0BNE5 Rat
- Unigene: 432491 Human
see all -
別名
- EC 3.1.2.12 antibody
- Es-10 antibody
- Es10 antibody
see all
画像
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All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaLanes 1-4: Merged signal (red and green). Green - ab133631 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133631 Anti-ESD antibody [EPR8447] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab133631 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ESD antibody [EPR8447] (ab133631) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab133631 は 2 報の論文で使用されています。
- Yang Y et al. Esterase D stabilizes FKBP25 to suppress mTORC1. Cell Mol Biol Lett 26:50 (2021). PubMed: 34875997
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233