Anti-SIM2 抗体 [EPR7878] (ab131161)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7878] to SIM2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SIM2 antibody [EPR7878]
SIM2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7878] to SIM2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2 whole cell lysate (ab7900); Human fetal forebrain and Human fetal brain lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7878 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 73 kDa). |
ターゲット情報
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機能
Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 Single-minded C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6493 Human
- Omim: 600892 Human
- SwissProt: Q14190 Human
- Unigene: 146186 Human
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別名
- bHLHe15 antibody
- Class E basic helix loop helix protein 15 antibody
- Class E basic helix-loop-helix protein 15 antibody
see all
画像
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Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SIM2 antibody [EPR7878] (ab131161) at 1/1000 dilution
Lane 1 : Human fetal forebrain lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab131161 は 1 報の論文で使用されています。
- Qin B et al. Long non-coding RNA CASC15 facilitates esophageal squamous cell carcinoma tumorigenesis via decreasing SIM2 stability via FTO-mediated demethylation. Oncol Rep 45:1059-1071 (2021). PubMed: 33650646