Anti-LMO4 抗体 [EPR6731(2)] (ab131030)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6731(2)] to LMO4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LMO4 antibody [EPR6731(2)]
LMO4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6731(2)] to LMO4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human LMO4 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- 293T, Jurkat, SH-SY5Y, and NIH3T3 cell lysates, 293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 7.90 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.13% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6731(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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IP |
1/20 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
IP
1/20 - 1/100. |
ターゲット情報
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機能
Probable transcriptional factor. -
配列類似性
Contains 2 LIM zinc-binding domains. - Information by UniProt
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参照データベース
- Entrez Gene: 8543 Human
- Entrez Gene: 16911 Mouse
- Omim: 603129 Human
- SwissProt: P61968 Human
- SwissProt: P61969 Mouse
- Unigene: 436792 Human
- Unigene: 480603 Mouse
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別名
- Breast tumor autoantigen antibody
- LIM domain only 4 antibody
- LIM domain only protein 4 antibody
see all
画像
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All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 17 kDa
Actual band size : 17 kDa -
All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney) whole cell lysate
Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
All lanes : Anti-LMO4 antibody [EPR6731(2)] (ab131030) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : Mouse cerebral cortex tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
Purified ab131030 at 1/20 immunoprecipitating LMO4 in Jurkat whole cell lysate observed at 18 KDa (lanes 1 and 2).
Lane 1 (input): Jurkat whole cell lysate 10ug
Lane 2 (+): ab131030 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab131030 in Jurkat whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking/Diluting buffer 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab131030 は 3 報の論文で使用されています。
- Jiang YM et al. CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis. Dis Markers 2021:3977189 (2021). PubMed: 34925640
- Xiong X et al. Long non-coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4. Oncol Rep 43:1503-1515 (2020). PubMed: 32323846
- Rosati R et al. Inhibition of protein nitration prevents cisplatin-induced inactivation of STAT3 and promotes anti-apoptotic signaling in organ of Corti cells. Exp Cell Res 381:105-111 (2019). PubMed: 31078568