Anti-PGD 抗体 [EPR6565] (ab129199)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6565] to PGD
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PGD antibody [EPR6565]
PGD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6565] to PGD -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PGD aa 400-500. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Raji, Molt-4, and 293T cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HEK293 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.53 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR6565 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa). |
ターゲット情報
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機能
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH. -
パスウェイ
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3. -
配列類似性
Belongs to the 6-phosphogluconate dehydrogenase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5226 Human
- Entrez Gene: 110208 Mouse
- Entrez Gene: 100360180 Rat
- Entrez Gene: 100363662 Rat
- Omim: 172200 Human
- SwissProt: P52209 Human
- SwissProt: Q9DCD0 Mouse
- SwissProt: P85968 Rat
see all -
別名
- 0610042A05Rik antibody
- 6 phosphogluconate dehydrogenase, decarboxylating antibody
- 6-phosphogluconate dehydrogenase antibody
see all
画像
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All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysates
Lane 2 : Mouse heart tissue lysates
Lane 3 : Mouse kidney tissue lysates
Lane 4 : Mouse spleen tissue lysates
Lane 5 : Rat brain tissue lysates
Lane 6 : Rat heart tissue lysates
Lane 7 : Rat kidney tissue lysates
Lane 8 : Rat spleen tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with ab129199 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
Overlay histogram showing HEK293 cells stained with ab129199 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129199, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab129199 は 9 報の論文で使用されています。
- Facchinello N et al. Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition. Nat Metab 4:123-140 (2022). PubMed: 35102339
- Gong J et al. The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates. JCI Insight 6:N/A (2021). PubMed: 33497360
- Tokuda K et al. Up-regulation of the pentose phosphate pathway and HIF-1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina. Cell Biol Int 44:137-144 (2020). PubMed: 31393075
- Xu K et al. HIF-1a regulates cellular metabolism, and Imatinib resistance by targeting phosphogluconate dehydrogenase in gastrointestinal stromal tumors. Cell Death Dis 11:586 (2020). PubMed: 32719331
- Tsachaki M et al. Impact of 17ß-HSD12, the 3-ketoacyl-CoA reductase of long-chain fatty acid synthesis, on breast cancer cell proliferation and migration. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302749
- Baardman J et al. A Defective Pentose Phosphate Pathway Reduces Inflammatory Macrophage Responses during Hypercholesterolemia. Cell Rep 25:2044-2052.e5 (2018). PubMed: 30463003
- Yoshida K et al. Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12). Metabolomics 13:98 (2017). PubMed: 28781589
- Bhanot H et al. Acute myeloid leukemia cells require 6-phosphogluconate dehydrogenase for cell growth and NADPH-dependent metabolic reprogramming. Oncotarget 8:67639-67650 (2017). PubMed: 28978059
- Rahman SM et al. The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer. JCI Insight 1:e88814 (2016). WB ; Human . PubMed: 27882349