Anti-MAT1A 抗体 [EPR7938] (ab129176)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7938] to MAT1A
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MAT1A antibody [EPR7938]
MAT1A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7938] to MAT1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Human fetal liver, Mouse liver, and Rat liver lysates. IP: 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 1.22 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7938 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129176の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
IP
1/10 - 1/100. |
ターゲット情報
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関連性
MAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. Mutations in this gene are associated with methionine adenosyltransferase deficiency. -
参照データベース
- Entrez Gene: 4143 Human
- Entrez Gene: 11720 Mouse
- Entrez Gene: 25331 Rat
- Omim: 610550 Human
- SwissProt: Q00266 Human
- SwissProt: Q91X83 Mouse
- SwissProt: P13444 Rat
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別名
- AdoMet antibody
- AdoMet synthetase 1 antibody
- AI046368 antibody
see all
画像
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ab129176 (Purified) at 1:40 dilution (2 µg) immunoprecipitating MAT1A in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab129176 & 293T whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/10000 dilution (Purified)
Lane 1 : Human fetal liver lysates
Lane 2 : Mouse liver lysates
Lane 3 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
All lanes : Anti-MAT1A antibody [EPR7938] (ab129176) at 1/1000 dilution (unpurified)
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab129176 は 4 報の論文で使用されています。
- Peng C et al. Cyclin H predicts the poor prognosis and promotes the proliferation of ovarian cancer. Cancer Cell Int 20:316 (2020). PubMed: 32694938
- Xu Q et al. HNF4a regulates sulfur amino acid metabolism and confers sensitivity to methionine restriction in liver cancer. Nat Commun 11:3978 (2020). PubMed: 32770044
- Aon MA et al. Untangling Determinants of Enhanced Health and Lifespan through a Multi-omics Approach in Mice. Cell Metab 32:100-116.e4 (2020). PubMed: 32413334
- Lapek JD et al. Defining Host Responses during Systemic Bacterial Infection through Construction of a Murine Organ Proteome Atlas. Cell Syst 6:579-592.e4 (2018). PubMed: 29778837