Anti-Citrate synthetase 抗体 [EPR8066] (ab129088)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8066] to Citrate synthetase
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Citrate synthetase antibody [EPR8066]
Citrate synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8066] to Citrate synthetase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- HepG2, Caco-2, 293T, and HeLa cell lysates, Human kidney tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8066 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Assay kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129088の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa).
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2. -
配列類似性
Belongs to the citrate synthase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 1431 Human
- Entrez Gene: 170587 Rat
- Omim: 118950 Human
- SwissProt: O75390 Human
- SwissProt: Q8VHF5 Rat
- Unigene: 430606 Human
- Unigene: 728168 Human
- Unigene: 66581 Rat
-
別名
- CISY_HUMAN antibody
- Citrate synthase antibody
- Citrate synthase, mitochondrial antibody
see all
画像
-
All lanes : Anti-Citrate synthetase antibody [EPR8066] (ab129088) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51 kDa -
ab129088, at a dilution of 1/100, staining Citrate synthetase in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab129088 は 2 報の論文で使用されています。
- Chen LY et al. Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Mol Med Rep 12:5035-41 (2015). WB ; Rat . PubMed: 26135512
- Zoladz JA et al. Mechanisms responsible for the acceleration of pulmonary V?O2 on-kinetics in humans after prolonged endurance training. Am J Physiol Regul Integr Comp Physiol 307:R1101-14 (2014). PubMed: 25163914