Anti-HNF-1B 抗体 [EPR6334(2)] (ab128912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6334(2)] to HNF-1B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HNF-1B antibody [EPR6334(2)]
HNF-1B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6334(2)] to HNF-1B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human HNF-1B aa 500-600. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human fetal kidney and Caco 2 lysates. ICC/IF: human, rat and mouse liver tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6334(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. -
関連疾患
Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy.
Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11) [MIM:611955]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. -
配列類似性
Belongs to the HNF1 homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6928 Human
- Entrez Gene: 21410 Mouse
- Entrez Gene: 25640 Rat
- Omim: 189907 Human
- SwissProt: P35680 Human
- SwissProt: P27889 Mouse
- SwissProt: P23899 Rat
- Unigene: 191144 Human
see all -
別名
- FJHN antibody
- Hepatocyte nuclear factor 1 beta antibody
- Hepatocyte nuclear factor 1-beta antibody
see all
画像
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Immunohistochemistry analysis of paraffin-embedded sections of rat liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry analysis of paraffin-embedded sections of human liver tissue staining HNF-1B wth ab128912 with 1/100 dilution (4.6 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
Immunohistochemistry analysis of paraffin-embedded sections of mouse liver tissue staining HNF-1B wth ab128912 with 1/2000 dilution (0.23 μg/mL concentration) and Ready-to-use secondary; counterstained with Hematoxylin. The section was incubated with ab128912 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
All lanes : Anti-HNF-1B antibody [EPR6334(2)] (ab128912) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab128912 は 2 報の論文で使用されています。
- Chan SC et al. Hepatocyte nuclear factor 1β suppresses canonical Wnt signaling through transcriptional repression of lymphoid enhancer-binding factor 1. J Biol Chem 295:17560-17572 (2020). PubMed: 33453998
- Liu Q et al. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960 (2018). PubMed: 30154523