Anti-Aquaporin 4 抗体 [EPR7040] (ab128906)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7040] to Aquaporin 4
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Aquaporin 4 antibody [EPR7040]
Aquaporin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7040] to Aquaporin 4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Aquaporin 4 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P55087 -
ポジティブ・コントロール
- WB: Mouse stomach, Mouse kidney, Rat stomach, Rat kidney and Mouse brain, Human stomach lysates (unboiled); IHC-P: Human cerebrum, Human stomach, Mouse cerebrum, Mouse cerebellum, Mouse stomach, Mouse kidney and Rat cerebrum, Rat stomach tissues. IHC-Fr: Rat stomach, Mouse stomach tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 2.60 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7040 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128906の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/100.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 35 kDa.
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特記事項 |
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IHC-Fr
1/100. |
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 35 kDa. |
ターゲット情報
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機能
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. -
組織特異性
Brain - muscle >> heart, kidney, lung, and trachea. -
配列類似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
ドメイン
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻訳後修飾
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 361 Human
- Entrez Gene: 11829 Mouse
- Entrez Gene: 25293 Rat
- Omim: 600308 Human
- SwissProt: P55087 Human
- SwissProt: P55088 Mouse
- SwissProt: P47863 Rat
- Unigene: 315369 Human
see all -
別名
- AQP 4 antibody
- AQP-4 antibody
- AQP4 antibody
see all
画像
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All lanes : Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution
Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100,28 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
We recommend not to boil the samples after lysis to get desired WB results.
The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
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All lanes : Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution
Lane 1 : Mouse kidney lysate unboiled
Lane 2 : Mouse brain lysate unboiled
Lane 3 : Rat stomach lysate unboiled
Lane 4 : Rat kidney lysate unboiled
Lane 5 : Human stomach lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 100,28 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST. 'Unboil method' will optimize WB result.
Exposure: Lane1 & Lane2: 20 seconds. Lane3 & Lane4 & Lane5: 120 seconds.
The molecular weight is consistent with what has been described in the literature. Bands observed at 100kDa relate to a tetramer of AQP4 (PMID:18334967, 11742978, 17897643).
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Anti-Aquaporin 4 antibody [EPR7040] (ab128906) at 1/1000 dilution + Human stomach lysate unboiled at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 28,30 kDa why is the actual band size different from the predicted?
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
'Unboil method' will optimize WB result.
The molecular weight is consistent with what has been described in the literature. (PMID: 22590566, 17897643).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 µg/mL) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach (fresh) tissue labeling Aquaporin 4 with ab128906 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat stomach. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on rat cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse kidney. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebellum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human stomach. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aquaporin 4 with ab128906 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Cytoplasmic staining on human cerebrum. The section was incubated with ab128906 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins
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ab128906 at 1/100 dilution, staining Aquaporin 4 in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab128906 は 11 報の論文で使用されています。
- Guan Y et al. Effect of AQP4-RNAi in treating traumatic brain edema: Multi-modal MRI and histopathological changes of early stage edema in a rat model. Exp Ther Med 19:2029-2036 (2020). PubMed: 32104262
- Kitchen P et al. Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell 181:784-799.e19 (2020). PubMed: 32413299
- Zhang H et al. Hypertonic saline improves brain edema resulting from traumatic brain injury by suppressing the NF-?B/IL-1ß signaling pathway and AQP4. Exp Ther Med 20:71 (2020). PubMed: 32963601
- Yu JZ et al. N-3 polyunsaturated fatty acids promote astrocyte differentiation and neurotrophin production independent of cAMP in patient-derived neural stem cells. Mol Psychiatry N/A:N/A (2020). PubMed: 32504049
- Barrera V et al. Neurovascular sequestration in paediatric P. falciparum malaria is visible clinically in the retina. Elife 7:N/A (2018). PubMed: 29578406