Anti-Themis 抗体 [EPR7353] (ab126771)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7353] to Themis
- Suitable for: IP, Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Themis antibody [EPR7353]
Themis 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7353] to Themis -
由来種
Rabbit -
アプリケーション
適用あり: IP, Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Themis aa 200-300. The exact sequence is proprietary.
Database link: Q8N1K5 -
ポジティブ・コントロール
- IP: Jurkat cells; WB: Jurkat and MOLT-4 cells, Mouse and rat thymus lysate; ICC/IF: Jurkat cells Flow Cyt (intra): Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7353 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126771の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
1/30.
|
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Flow Cyt (Intra) |
1/50.
For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 73 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
ICC/IF |
1/50.
|
特記事項 |
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IP
1/30. |
Flow Cyt (Intra)
1/50. For unpurified use at 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 73 kDa. For unpurified use at 1/1000 - 1/10000. |
ICC/IF
1/50. |
ターゲット情報
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機能
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. -
配列類似性
Belongs to the themis family. -
翻訳後修飾
Phosphorylated on Tyr residues quickly after TCR stimulation. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 387357 Human
- Entrez Gene: 210757 Mouse
- Omim: 613607 Human
- SwissProt: Q8N1K5 Human
- SwissProt: Q8BGW0 Mouse
- Unigene: 661756 Human
- Unigene: 123021 Mouse
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別名
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
see all
画像
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Purified ab126771 at 1/30 dilution (2µg) immunoprecipitating Themis in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab126771 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126771 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 73 kDa -
All lanes : Anti-Themis antibody [EPR7353] (ab126771) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : Mouse thymus lysate
Lane 4 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaThe molecular weight observed is consistent with what has been described in the literature (PMID: 25535246 and PMID: 19597499).
Blocking Buffer and concentration: 5% NFDM/TBST
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Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Themis with Purified ab126771 at 1:50 dilution (10 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Themis with Purified ab126771 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab126771 は 2 報の論文で使用されています。
- Lyu J et al. Thymic-specific regulation of TCR signaling by Tespa1. Cell Mol Immunol 16:897-907 (2019). PubMed: 31316154
- Kinosada H et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog 13:e1006120 (2017). IF ; Mouse . PubMed: 28046066