Anti-LPP 抗体 [EPR6478] (ab126608)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6478] to LPP
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LPP antibody [EPR6478]
LPP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6478] to LPP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Caco 2, HeLa, NIH 3T3 cell lysates; Human breast and kidney tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.60 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6478 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126608の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus. -
組織特異性
Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. -
関連疾患
Note=A chromosomal aberration involving LPP is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with HMGA2 is shown in lipomas.
Note=A chromosomal aberration involving LPP is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with HMGA2 is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving LPP is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with HMGA2 is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving LPP is associated with acute monoblastic leukemia. Translocation t(3;11)(q28;q23) with MLL is associated with acute monoblastic leukemia. -
配列類似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
細胞内局在
Nucleus. Cytoplasm. Cell junction. Cell membrane. Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and MLL. - Information by UniProt
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参照データベース
- Entrez Gene: 4026 Human
- Entrez Gene: 210126 Mouse
- Entrez Gene: 288010 Rat
- Omim: 600700 Human
- SwissProt: Q93052 Human
- SwissProt: Q8BFW7 Mouse
- SwissProt: Q5XI07 Rat
- Unigene: 5724 Human
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別名
- DKFZp779O0231 antibody
- FLJ30652 antibody
- FLJ41512 antibody
see all
画像
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All lanes : Anti-LPP antibody [EPR6478] (ab126608) at 1/10000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 66 kDa -
ab122608 at 1/250 staining LPP in paraffin embedded Human breast tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab122608 at 1/250 staining LPP in paraffin embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab126608 は 2 報の論文で使用されています。
- Wang H et al. Fibroblast-derived LPP as a biomarker for treatment response and therapeutic target in gastric cancer. Mol Ther Oncolytics 24:547-560 (2022). PubMed: 35229032
- Ngan E et al. LPP is a Src substrate required for invadopodia formation and efficient breast cancer lung metastasis. Nat Commun 8:15059 (2017). IHC-P ; Mouse . PubMed: 28436416