Anti-GRO alpha + GRO gamma + GRO beta 抗体 [EPR4902] (ab124898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4902] to CXCL1/GRO alpha + GRO gamma + GRO beta
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902]
CXCL1/GRO alpha+GRO gamma+GRO beta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4902] to CXCL1/GRO alpha + GRO gamma + GRO beta -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human GRO alpha aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human GRO alpha recombinant protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4902 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124898の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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細胞内局在
CXCL1/GRO alpha: Secreted. GRO gamma: Secreted. -
参照データベース
- Entrez Gene: 2919 Human
- Entrez Gene: 2921 Human
- Omim: 139111 Human
- Omim: 155730 Human
- SwissProt: P09341 Human
- SwissProt: P19875 Human
- SwissProt: P19876 Human
- Unigene: 708652 Human
see all
画像
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All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,36,10 kDa why is the actual band size different from the predicted?Exposure time: 3 minutes
Blocking buffer and concentartion:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Human GRO beta fragment protein contain aa35-107 with a GST-Tage.Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124898 は 1 報の論文で使用されています。
- Pasquier J et al. Circulating microparticles in acute diabetic Charcot foot exhibit a high content of inflammatory cytokines, and support monocyte-to-osteoclast cell induction. Sci Rep 7:16450 (2017). PubMed: 29180664