Anti-Mitochondrial Ferritin 抗体 [EPR1797] (ab124889)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1797] to Mitochondrial Ferritin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mitochondrial Ferritin antibody [EPR1797]
Mitochondrial Ferritin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1797] to Mitochondrial Ferritin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Mitochondrial Ferritin aa 200-300 (C terminal). The exact sequence is proprietary.
Database link: Q8N4E7 -
ポジティブ・コントロール
- Human Testis lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1797 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124889の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 28 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. -
組織特異性
Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine. -
配列類似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 94033 Human
- Omim: 608847 Human
- SwissProt: Q8N4E7 Human
- Unigene: 105324 Human
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別名
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Positve staining on the human testis. The section was incubated with ab124889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Negative control: no staining on the human cerebrum. The section was incubated with ab124889 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: human cerebellum.
In Western blot, anti-Anti-beta Tubulin antibody (ab52901) staining at 1/20000 dilution. -
Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution + Human Testis lysate at 10 µg
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab124889 は 4 報の論文で使用されています。
- Munuera-Cabeza M et al. Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A Syndrome. Genes (Basel) 13:N/A (2022). PubMed: 36553567
- Gong S et al. Roflumilast enhances cisplatin-sensitivity and reverses cisplatin-resistance of ovarian cancer cells via cAMP/PKA/CREB-FtMt signalling axis. Cell Prolif 51:e12474 (2018). PubMed: 30069985
- Gong S et al. Roflumilast restores cAMP/PKA/CREB signaling axis for FtMt-mediated tumor inhibition of ovarian cancer. Oncotarget 8:112341-112353 (2017). PubMed: 29348829
- Shi ZH et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . PubMed: 25213357