Anti-EPS8 抗体 [EPR6112] (ab124882)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6112] to EPS8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EPS8 antibody [EPR6112]
EPS8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6112] to EPS8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human EPS8 aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa, A375, 293T, and K562 cell lysates, Human kidney tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6112 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124882の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
ターゲット情報
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機能
Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. -
組織特異性
Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. -
配列類似性
Belongs to the EPS8 family.
Contains 1 PH domain.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylated by several receptor tyrosine kinases. - Information by UniProt
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参照データベース
- Entrez Gene: 2059 Human
- Omim: 600206 Human
- SwissProt: Q12929 Human
- Unigene: 591160 Human
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別名
- Epidermal growth factor receptor kinase substrate 8 antibody
- Epidermal growth factor receptor pathway substrate 8 antibody
- EPS 8 antibody
see all
画像
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All lanes : Anti-EPS8 antibody [EPR6112] (ab124882) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124882 は 2 報の論文で使用されています。
- Lu H et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 14:4999-5006 (2016). WB ; Human . PubMed: 27840923
- Tang B et al. Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 12:1741-52 (2015). PubMed: 25936538