Anti-BRAF (phospho S729) 抗体 [EPR2207] (ab124794)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2207] to BRAF (phospho S729)
- Suitable for: WB
- Reacts with: Rat
Related conjugates and formulations
製品の概要
-
製品名
Anti-BRAF (phospho S729) antibody [EPR2207]
BRAF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2207] to BRAF (phospho S729) -
由来種
Rabbit -
特異性
Detects B Raf only when phosphorylated on serine 729. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human BRAF.
-
ポジティブ・コントロール
- PC-12 cell lysates
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 9.00 x 10 -12 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2207 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124794の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa. |
ターゲット情報
-
機能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. -
組織特異性
Brain and testis. -
関連疾患
Note=Defects in BRAF are found in a wide range of cancers.
Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. - Information by UniProt
-
参照データベース
- Entrez Gene: 673 Human
- Entrez Gene: 109880 Mouse
- Entrez Gene: 114486 Rat
- Omim: 164757 Human
- SwissProt: P15056 Human
- SwissProt: P28028 Mouse
- Unigene: 550061 Human
- Unigene: 245513 Mouse
-
別名
- FLJ95109 antibody
- 94 kDa B raf protein antibody
- B raf 1 antibody
see all
画像
-
All lanes : Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794) at 1/1000 dilution
Lane 1 : PC-12 cell lysates (untreated)
Lane 2 : PC-12 cell lysates treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab124794 は 8 報の論文で使用されています。
- Oberoi J et al. HSP90-CDC37-PP5 forms a structural platform for kinase dephosphorylation. Nat Commun 13:7343 (2022). PubMed: 36446791
- Cope NJ et al. Analyses of the oncogenic BRAFD594G variant reveal a kinase-independent function of BRAF in activating MAPK signaling. J Biol Chem 295:2407-2420 (2020). PubMed: 31929109
- Zhang Y et al. Glycyrrhetinic acid binds to the conserved P-loop region and interferes with the interaction of RAS-effector proteins. Acta Pharm Sin B 9:294-303 (2019). PubMed: 30976491
- Wang X et al. AMPK Promotes SPOP-Mediated NANOG Degradation to Regulate Prostate Cancer Cell Stemness. Dev Cell 48:345-360.e7 (2019). PubMed: 30595535
- Fujita K et al. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. Nat Commun 9:433 (2018). PubMed: 29382817
- Vido MJ et al. BRAF Splice Variant Resistance to RAF Inhibitor Requires Enhanced MEK Association. Cell Rep 25:1501-1510.e3 (2018). PubMed: 30404005
- Gantois I et al. Metformin ameliorates core deficits in a mouse model of fragile X syndrome. Nat Med 23:674-677 (2017). PubMed: 28504725
- Guo Y et al. Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis. Cancer Res 76:773-86 (2016). PubMed: 26676752