Spermidine hydrochloride, nNOS inhibitor (ab120057)
Key features and details
- Endogenous polyamine. nNOS inhibitor.
- CAS Number: 334-50-9
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Spermidine hydrochloride, nNOS inhibitor -
製品の詳細
Endogenous polyamine. nNOS inhibitor. -
生理活性の詳細
Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
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CAS 番号
334-50-9 -
構造式
製品の特性
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体系名
N-(3-Aminopropyl)butane-1,4-diamine trihydrochloride -
分子量
254.63 -
分子式
C7H19N3.3HCl -
PubChem 登録番号
9539 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
NCCCNCCCCN.Cl.Cl.Cl -
由来
Synthetic
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研究分野
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Stroke
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120057の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120057, Spermidine hydrochloride, nNOS inhibitor
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ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120057 は 1 報の論文で使用されています。
- Ullrich M et al. Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans. Nat Protoc 9:2237-55 (2014). PubMed: 25167056