Recombinant human TNIK protein (ab119725)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human TNIK protein -
生理活性
ab119725 specific activity determined to be 68 nmol/min/mg.
-
精製度
> 95 % SDS-PAGE.
The purity was determined to be 95% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
67 kDa including tags -
領域
1 to 367 -
タグ
GST tag N-Terminus
-
関連製品
-
Substrate reagent
特性
Our Abpromise guarantee covers the use of ab119725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Western blot
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.003% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- EC 2.7.11.1
- KIAA0551
- Tnik
see all -
機能
Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. -
組織特異性
Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CNH domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction. -
細胞内局在
Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression. - Information by UniProt
画像
-
The specific activity of TNIK (ab119725) was determined to be 50 nmol/min/mg as per activity assay protocol and was equivalent to 58 nmol/min/mg as per radiometric assay
-
SDS PAGE analysis of ab119725
-
SDS PAGE analysis of ab119725
-
SDS Page analysis of ab119725
-
ab119725 specific activity determined to be 68 nmol/min/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab119725 は論文での使用が確認できていません。