Mouse TGF beta 1 ELISA Kit (ab119557)
Key features and details
- Sensitivity: 7.8 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse TGF beta 1 ELISA Kit
TGF beta 1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4 7.9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 4 5.8% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
7.8 pg/ml -
検出範囲
31.3 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 124% - 141% Serum 92% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 20X Assay Buffer Concentrate 2 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 6 units Biotinylated mouse anti-TGF beta 1 monoclonal antibody 1 x 120µl Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit Mouse TGF beta 1 Standard lyophilized 2 vials Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究分野
-
機能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
組織特異性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
関連疾患
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
別名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
参照データベース
- Entrez Gene: 21803 Mouse
- SwissProt: P04202 Mouse
- Unigene: 248380 Mouse
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (49)
ab119557 は 49 報の論文で使用されています。
- O'Brien A et al. FGF1 Signaling Modulates Biliary Injury and Liver Fibrosis in the Mdr2-/- Mouse Model of Primary Sclerosing Cholangitis. Hepatol Commun 6:1574-1588 (2022). PubMed: 35271760
- Dai S et al. Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway. J Neuroinflammation 19:122 (2022). PubMed: 35624490
- Ma L et al. Lactobacillus improves the effects of prednisone on autoimmune hepatitis via gut microbiota-mediated follicular helper T cells. Cell Commun Signal 20:83 (2022). PubMed: 35658901
- Yang X et al. Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis. Stem Cell Res Ther 13:279 (2022). PubMed: 35765096
- Xiu W et al. Potentiating hypoxic microenvironment for antibiotic activation by photodynamic therapy to combat bacterial biofilm infections. Nat Commun 13:3875 (2022). PubMed: 35790729