Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric) (ab115093)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 5 ng/well
製品の概要
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製品名
Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric)
Histone H4 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
検出感度
5 ng/well -
検出範囲
20 ng/well - 2000 ng/well -
全工程の試験時間
2h 30m -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Mammals -
製品の概要
Methylation of histone H4 at lysine 20 is catalyzed by SET9 and SUV4.20h among other methyl transferases in mammalian cells. H4 (mono-methyl K20) is involved in the maintenance of the proper higher order structure of DNA and is consequently essential for chromosome condensation. It is also involved in gene silencing.
Histone H4 (mono-methyl K20) Quantification Kit (Colorimetric) (ab115093) enables the user to measure global mono-methylation of histone H4K20. The global H4K20 mono-methylation can also be changed by inhibition or activation of HMTs, making quantitative detection of global mono-methyl histone H4K20 useful in developing a better understanding of epigenetic regulation of gene activation/repression and for developing HMT-targeted drugs. ab115093 is suitable for use with a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 ラベル 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with Frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detecting Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Signal Enhancer 1 x 120µl 1 x 240µl Signal Report Solution 1 x 5µl 1 x 10µl Standard Control, 100 µg/mL 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
研究分野
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H4 family. -
翻訳後修飾
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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別名
- dJ160A22.1
- dJ160A22.2
- dJ221C16.1
see all -
参照データベース
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8368 Human
- Entrez Gene: 8370 Human
see all
関連製品
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Related Products
- Histone H4 (mono-methyl K20) Quantification Kit (Fluorometric) (ab115094)
- Histone H4 (di-methyl K20) Quantification Kit (Colorimetric) (ab115095)
- Histone H4 (di-methyl K20) Quantification Kit (Fluorometric) (ab115096)
- Histone H4 (tri-methyl K20) Quantification Kit (Colorimetric) (ab115097)
- Histone H4 (tri-methyl K20) Quantification Kit (Fluorometric) (ab115098)
- Histone H4 (pan-methyl K20) Quantification Kit (Colorimetric) (ab115099)
- Histone H4 (pan-methyl K20) Quantification Kit (Fluorometric) (ab115100)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115093 は論文での使用が確認できていません。