Anti-GRB7 抗体 [EPR4378] (ab109618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4378] to GRB7
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-GRB7 antibody [EPR4378]
GRB7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4378] to GRB7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: SKBR-3, T-47D, MCF7, HEPG2 and HeLa cell lysates. IHC-P: Human breast tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4378 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109618の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/200 - 1/1000. Predicted molecular weight: 60 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat to 98°C, allow to cool for 10-20 minutes. |
特記事項 |
---|
WB
1/200 - 1/1000. Predicted molecular weight: 60 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat to 98°C, allow to cool for 10-20 minutes. |
ターゲット情報
-
機能
Interacts with the cytoplasmic domain of the epidermal growth factor receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds to ERBB2. -
配列類似性
Belongs to the GRB7/10/14 family.
Contains 1 PH domain.
Contains 1 Ras-associating domain.
Contains 1 SH2 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues. - Information by UniProt
-
参照データベース
- Entrez Gene: 2886 Human
- Entrez Gene: 14786 Mouse
- Entrez Gene: 84427 Rat
- Omim: 601522 Human
- SwissProt: Q14451 Human
- SwissProt: Q03160 Mouse
- SwissProt: Q9QZC5 Rat
- Unigene: 86859 Human
see all -
別名
- B47 antibody
- Epidermal growth factor receptor GRB 7 antibody
- Epidermal growth factor receptor GRB-7 antibody
see all
画像
-
All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/200 dilution (Purified)
Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
The expression level of GRB7 in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID: 7907978 and 17916906.
-
ab109618 at 1/50 dilution staining GRB7 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-GRB7 antibody [EPR4378] (ab109618) at 1/1000 dilution
Lane 1 : SKBR-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 60 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab109618 は 1 報の論文で使用されています。
- Atsumi S et al. Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases. Commun Biol 3:588 (2020). PubMed: 33067514