Anti-Proteasome subunit alpha type 6 抗体 [EPR5835] (ab109377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Proteasome subunit alpha type 6 antibody [EPR5835]
Proteasome subunit alpha type 6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, 293T, HeLa and Jurkat cell lysates IHC-P: Human colon tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5835 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109377の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
特記事項 |
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WB
1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
ターゲット情報
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機能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
配列類似性
Belongs to the peptidase T1A family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5687 Human
- Entrez Gene: 26443 Mouse
- Entrez Gene: 29673 Rat
- Omim: 602855 Human
- SwissProt: P60900 Human
- SwissProt: Q9QUM9 Mouse
- SwissProt: P60901 Rat
- Unigene: 446260 Human
see all -
別名
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
see all
画像
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All lanes : Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa -
ab109377, at a 1/250 dilution, staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab109377 は 7 報の論文で使用されています。
- Zhang W et al. The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation. Gene 769:145228 (2021). PubMed: 33096182
- Mishra M et al. Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP). Electrophoresis N/A:N/A (2019). PubMed: 31021001
- Sander H et al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem 575:44-53 (2019). PubMed: 30914243
- Zhu Y et al. Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network. Oncotarget 8:2233-2248 (2017). WB ; Human . PubMed: 27903980
- Ghosh R et al. Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts. Int J Cardiol 223:923-935 (2016). WB ; Mouse . PubMed: 27589040
- Gilda JE et al. Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS One 10:e0135392 (2015). PubMed: 26287535
- Ando R et al. Decreased proteasomal activity causes photoreceptor degeneration in mice. Invest Ophthalmol Vis Sci 55:4682-90 (2014). Mouse . PubMed: 24994871