Anti-Frizzled 9 抗体 [EPR4011] (ab108628)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4011] to Frizzled 9
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Frizzled 9 antibody [EPR4011]
Frizzled 9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4011] to Frizzled 9 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human seminoma, fetal kidney and fetal testis lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4011 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108628の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 64 kDa). |
ターゲット情報
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機能
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
組織特異性
Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney. -
配列類似性
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
ドメイン
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8326 Human
- Entrez Gene: 14371 Mouse
- Entrez Gene: 266608 Rat
- Omim: 601766 Human
- SwissProt: O00144 Human
- SwissProt: Q9R216 Mouse
- Unigene: 647029 Human
- Unigene: 6256 Mouse
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別名
- CD 349 antibody
- CD 349 antigen antibody
- CD349 antibody
see all
画像
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All lanes : Anti-Frizzled 9 antibody [EPR4011] (ab108628) at 1/1000 dilution
Lane 1 : Human Seminoma lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108628 は 2 報の論文で使用されています。
- Rogan MR et al. Ehrlichia chaffeensis TRP120 Is a Wnt Ligand Mimetic That Interacts with Wnt Receptors and Contains a Novel Repetitive Short Linear Motif That Activates Wnt Signaling. mSphere 6:N/A (2021). PubMed: 33883266
- Shin W et al. Human Intestinal Morphogenesis Controlled by Transepithelial Morphogen Gradient and Flow-Dependent Physical Cues in a Microengineered Gut-on-a-Chip. iScience 15:391-406 (2019). PubMed: 31108394