Human IL-9 ELISA Kit (ab100577)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.04 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-9 ELISA Kit
IL-9 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.1 ng/ml -
検出範囲
0.04 ng/ml - 30 ng/ml -
添加回収試験
98 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 102.1 90% - 110% Plasma 82.4 67% - 98% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s IL-9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human IL-9 in normal serum with ab100577, only in serum spiked with Human IL-9.
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特記事項
Optimization may be required with urine samples.
The standard is affinity purified.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-9 2 vials IL-9 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
配列類似性
Belongs to the IL-7/IL-9 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Cytokine P40
- Homolog of mouse T cell and mast cell growth factor 40
- HP40
see all -
参照データベース
- Entrez Gene: 3578 Human
- Omim: 146931 Human
- SwissProt: P15248 Human
- Unigene: 960 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab100577 は論文での使用が確認できていません。