Human DKK1 ELISA Kit (Dickkopf-1) (ab100501)
Key features and details
- Sensitivity: 100 pg/ml
- Range: 122.9 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human DKK1 ELISA Kit (Dickkopf-1)
DKK1 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 100 pg/ml -
検出範囲
122.9 pg/ml - 30000 pg/ml -
添加回収試験
90 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 108.3 93% - 123% Serum 92.3 62% - 130% Plasma 72.8 59% - 110% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human DKK1 2 vials DKK1 Microplate (12 x 8 wells) 1 unit Recombinant Human DKK1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
DKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. -
別名
- Dickkopf 1
- Dickkopf homolog 1
- Dickkopf related protein 1
see all -
参照データベース
- Entrez Gene: 22943 Human
- Omim: 605189 Human
- SwissProt: O94907 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab100501 は 4 報の論文で使用されています。
- Ponzetti M et al. Lipocalin 2 increases after high-intensity exercise in humans and influences muscle gene expression and differentiation in mice. J Cell Physiol 237:551-565 (2022). PubMed: 34224151
- Caldwell C et al. Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors. Sci Rep 11:9920 (2021). PubMed: 33972574
- Xiong JH et al. Clinical significance and prognostic value of tumor necrosis factor-a and dickkopf related protein-1 in ankylosing spondylitis. World J Clin Cases 8:1213-1222 (2020). PubMed: 32337195
- Wang H et al. Priming integrin a5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity. J Cell Physiol N/A:N/A (2018). PubMed: 30556904