Anti-AANAT (phospho T29) 抗体 (ab3439)

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Key features and details

  • Rabbit polyclonal to AANAT (phospho T29)
  • Suitable for: WB
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-AANAT (phospho T29) antibody
    AANAT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to AANAT (phospho T29)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Rat
    交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey

  • 免疫原

    Synthetic peptide corresponding to Mouse AANAT aa 24-33 (N terminal) (phospho T29).
    Sequence: CQRRHTpLPAS
    (Peptide available as ab4975)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Rat pineal gland whole protein extract.

  • 特記事項

     This product was previously labelled as Serotonin N-acetyltransferase

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

  • 一次抗体 備考

    Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

ターゲット情報

  • 機能

    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

  • 組織特異性

    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

  • パスウェイ

    Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

  • 関連疾患

    Delayed sleep phase syndrome

  • 配列類似性

    Belongs to the acetyltransferase family. AANAT subfamily.
    Contains 1 N-acetyltransferase domain.

  • 翻訳後修飾

    cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AA-NAT antibody
    • Aanat antibody
    • Aralkylamine N-acetyltransferase antibody
    • Arylalkylamine N acetyltransferase antibody
    • Serotonin acetylase antibody
    • Serotonin N-acetyltransferase antibody
    • SNAT antibody
    • SNAT_HUMAN antibody
    see all

画像

  • Western blot - Anti-AANAT (phospho T29) antibody (ab3439)
    Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

    Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

参考文献 (2)

ab3439 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3439 は 2 報の論文で使用されています。

  • Hou B  et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
  • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074

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