Key features and details
- Rabbit polyclonal to AANAT (phospho T29)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品名Anti-AANAT (phospho T29) antibody
AANAT 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to AANAT (phospho T29)
アプリケーション適用あり: WBmore details
交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey
- Rat pineal gland whole protein extract.
This product was previously labelled as Serotonin N-acetyltransferase
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.
一次抗体 備考Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.
Our Abpromise guarantee covers the use of ab3439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.
機能Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
組織特異性Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
パスウェイAromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.
関連疾患Delayed sleep phase syndrome
配列類似性Belongs to the acetyltransferase family. AANAT subfamily.
Contains 1 N-acetyltransferase domain.
翻訳後修飾cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.
- Information by UniProt
- AA-NAT antibody
- Aanat antibody
- Aralkylamine N-acetyltransferase antibody
ab3439 は 2 報の論文で使用されています。
- Hou B et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
- Moran AW et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074