Anti-AANAT (phospho T29) 抗体 (ab3439)

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Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

    Key features and details

    • Rabbit polyclonal to AANAT (phospho T29)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-AANAT (phospho T29) antibody
      AANAT 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to AANAT (phospho T29)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Chicken, Hamster, Chimpanzee, Rhesus monkey

    • 免疫原

      Synthetic peptide corresponding to Mouse AANAT aa 24-33 (N terminal) (phospho T29).
      Sequence: CQRRHTpLPAS
      (Peptide available as ab4975)

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Rat pineal gland whole protein extract.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

    • 一次抗体 備考

      Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3439の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

    By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.
    特記事項
    WB
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

    By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

    ターゲット情報

    • 機能

      Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

    • 組織特異性

      Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

    • パスウェイ

      Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

    • 関連疾患

      Delayed sleep phase syndrome

    • 配列類似性

      Belongs to the acetyltransferase family. AANAT subfamily.
      Contains 1 N-acetyltransferase domain.

    • 翻訳後修飾

      cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AA-NAT antibody
      • Aanat antibody
      • Aralkylamine N-acetyltransferase antibody
      • Arylalkylamine N acetyltransferase antibody
      • Serotonin acetylase antibody
      • Serotonin N-acetyltransferase antibody
      • SNAT antibody
      • SNAT_HUMAN antibody
      see all

    画像

    • Western blot - Anti-AANAT (phospho T29) antibody (ab3439)
      Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

      Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

    参考文献 (2)

    ab3439 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3439 は 2 報の論文で使用されています。

    • Hou B  et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
    • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074

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