Anti-AANAT (phospho T29) 抗体 (ab3439)

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Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

    Key features and details

    • Rabbit polyclonal to AANAT (phospho T29)
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-AANAT (phospho T29) antibody
      AANAT 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to AANAT (phospho T29)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat
      交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey

    • 免疫原

      Synthetic peptide corresponding to Mouse AANAT aa 24-33 (N terminal) (phospho T29).
      Sequence: CQRRHTpLPAS
      (Peptide available as ab4975)

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Rat pineal gland whole protein extract.

    • 特記事項

       This product was previously labelled as Serotonin N-acetyltransferase

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific N-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

    • 一次抗体 備考

      Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab3439 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

    By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract.

    ターゲット情報

    • 機能

      Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

    • 組織特異性

      Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

    • パスウェイ

      Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

    • 関連疾患

      Delayed sleep phase syndrome

    • 配列類似性

      Belongs to the acetyltransferase family. AANAT subfamily.
      Contains 1 N-acetyltransferase domain.

    • 翻訳後修飾

      cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AA-NAT antibody
      • Aanat antibody
      • Aralkylamine N-acetyltransferase antibody
      • Arylalkylamine N acetyltransferase antibody
      • Serotonin acetylase antibody
      • Serotonin N-acetyltransferase antibody
      • SNAT antibody
      • SNAT_HUMAN antibody
      see all

    画像

    • Western blot - Anti-AANAT (phospho T29) antibody (ab3439)
      Western blot - Anti-AANAT (phospho T29) antibody (ab3439)

      Western blot detection of Serotonin N-acetyltransferase (phospho T29) in transfected 293T cell lysate using ab3439.

    参考文献 (2)

    ab3439 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3439 は 2 報の論文で使用されています。

    • Hou B  et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). IHC (PFA fixed) ; Rat . PubMed: 28473754
    • Moran AW  et al. Expression of Na+/glucose co-transporter 1 (SGLT1) is enhanced by supplementation of the diet of weaning piglets with artificial sweeteners. Br J Nutr 104:637-46 (2010). PubMed: 20338074

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