Anti-AANAT 抗体 (ab3505)

Submit a review Q&A (1)References (4)

Key features and details

  • Rabbit polyclonal to AANAT
  • Suitable for: WB
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-AANAT antibody
    AANAT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to AANAT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Rat
    交差が予測される動物種: Mouse, Chicken, Hamster, Human, Chimpanzee, Rhesus monkey

  • 免疫原

    Synthetic peptide corresponding to Mouse AANAT aa 24-33 (N terminal).
    Sequence:

    CQRRHTLPAS


    (Peptide available as ab5003)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    .

     This product was previously labelled as Serotonin N-acetyltransferase

     

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Total anti-N-terminal AA-NAT-specific IgG was affinity purified on resin-coupled non-phosphopeptide. Phospho-specific amino-terminal IgG was subsequently isolated on resin-coupled phosphopeptide.

  • 一次抗体 備考

    Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light/dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).Can be blocked with AANAT peptide (ab5003).

This antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract.

ターゲット情報

  • 機能

    Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

  • 組織特異性

    Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

  • パスウェイ

    Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.

  • 関連疾患

    Delayed sleep phase syndrome

  • 配列類似性

    Belongs to the acetyltransferase family. AANAT subfamily.
    Contains 1 N-acetyltransferase domain.

  • 翻訳後修飾

    cAMP-dependent phosphorylation on both N-terminal Thr-31 and C-terminal Ser-205 regulates AANAT activity by promoting interaction with 14-3-3 proteins.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AA-NAT antibody
    • Aanat antibody
    • Aralkylamine N-acetyltransferase antibody
    • Arylalkylamine N acetyltransferase antibody
    • Serotonin acetylase antibody
    • Serotonin N-acetyltransferase antibody
    • SNAT antibody
    • SNAT_HUMAN antibody
    see all

参考文献 (4)

ab3505 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3505 は 4 報の論文で使用されています。

  • Wu D  et al. Loading-induced antitumor capability of murine and human urine. FASEB J 34:7578-7592 (2020). PubMed: 32293076
  • Chen L  et al. Pinealectomy or light exposure exacerbates biliary damage and liver fibrosis in cholestatic rats through decreased melatonin synthesis. Biochim Biophys Acta Mol Basis Dis 1865:1525-1539 (2019). PubMed: 30890428
  • Hou B  et al. Homeostatic Plasticity Mediated by Rod-Cone Gap Junction Coupling in Retinal Degenerative Dystrophic RCS Rats. Front Cell Neurosci 11:98 (2017). WB, IHC (PFA fixed) ; Rat . PubMed: 28473754
  • Alkozi HA  et al. TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein Level. Int J Mol Sci 18:N/A (2017). WB, IHC-Fr ; Human . PubMed: 28368307

レビューと Q&A

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Question

The customer has tested the secondary with other primary antibody, it's proved to work well. and she has got postive band on the same membrane when using actin antibody(loading control?, this proved that the load amount of the protein is enough. about block with 5%BSA, the customer and we think it shouldn't affect the result so much, so the custmer wouldn't like to retest it again. the cell lysate from rat pineal cells the customer used are from primary cell culture and the cells were collected after cultured for 10 days. the cells has been proved still show pineal cell characteristics by detected with 5-HT and melatonin in the culture supernate.

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Answer

Regarding the feedback on the secondary and the loading control working well, this is very reassuring and tells us the problem is not due to these. As the customer is sure the cells she is investigating are still pineal and should definitely express Serotonin N-acetyltransferase we would indeed expect a band at the correct size in her samples. I have read through Tanya's recommendations and agree with her that blocking can make a big difference. In our laboratory we try various blocking agents and see significant changes in the binding ability of antibodies. We have had instances where milk has prevented the binding of an antibody but where BSA gave very a good signal. Please pass on all our technical recommendations to our customers as if they do not follow our recommendations we will not offer replacements or refunds if the antibody was supposed to work as described on the datasheet. I would therefore appreciate you asking the customer to try with a different blocking agent of 5% BSA for 1hr (incubating the antibody in TBST only). If the customer still experiences problems with this modification please do not hesitate to contact us again,

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