製品名A-431 nuclear extract lysate (EGF treated)
A431 ライゼート 製品一覧
特記事項Cell line: A431 (Human epithelial carcinoma).
Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
We recommend aliquoting the extracts into single use fractions and then storing.
保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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背景A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.