Anti-WWP1 抗体 (ab104440)
Key features and details
- Rabbit polyclonal to WWP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WWP1 antibody
WWP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WWP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee -
免疫原
Synthetic peptide, corresponding to a region between amino acids 200 - 250 of Human WWP1 (NP_008944.1).
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ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab104440 is affinity purified using an epitope specific to WWP1 immobilized on solid support -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers. -
細胞内局在
Cell Membrane, Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 11059 Human
- Omim: 602307 Human
- SwissProt: Q9H0M0 Human
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別名
- AIP 5 antibody
- AIP5 antibody
- Atropin-1-interacting protein 5 antibody
see all
画像
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All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds -
Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.
Detection by chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab104440 は 1 報の論文で使用されています。
- Yi Y et al. Metformin Promotes AMP-activated Protein Kinase-independent Suppression of ?Np63a Protein Expression and Inhibits Cancer Cell Viability. J Biol Chem 292:5253-5261 (2017). PubMed: 28193839