製品の概要

  • 製品名
    Anti-WDR41 antibody
  • 製品の詳細
    Rabbit polyclonal to WDR41
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 252-282 of Human WDR41 (NP_060738.2).

  • ポジティブ・コントロール
    • Mouse cerebellum tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 52 kDa.

ターゲット情報

画像

  • Anti-WDR41 antibody (ab108096) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size: 52 kDa

プロトコール

参考文献

This product has been referenced in:
  • Jung J  et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28195531) »
See 1 Publication for this product

レビューと Q&A

Question

A few weeks ago we sent you this message after learning that you were having difficulty with one of our products. I wanted to follow up in case you did not receive this email.

We would like to offer our assistance, but we need to know more protocol information for us to understand the difficulty with the antibody. Please fill out the details below so that we may take a closer look. If the antibody is being used in accordance with the Abpromise, we may be able to offer a replacement or refund within 6 months of the purchase.

1) Abcam product code ab108096

2) Abcam order reference number or product batch number

3) Description of the problem

Multiple bands and none of them are correct.

4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein)
whole cell lysate from mouse brain and HeLa cell extract

Lysis buffer
SDS-PAGE sample buffer

Protease inhibitors:
None.

Phosphatase inhibitors
None.

Reducing agent
DTT

Boiling for 5 min? yes/no
Yes

Protein loaded ug/lane or cells/lane
50 ug

Positive control
whole cell extract.

Negative control
None.

5) Percentage of gel
4˜12% gradient gel

Type of membrane
nitrocellulose

Protein transfer verified
prestain protein marker and ponceau-S staining after transfer

Blocking agent and concentration
5% milk in TBST

Blocking time
1 hour

Blocking temperature
room temp.

6) Primary antibody
1:100, 1:250, 1:500, 1:1000

Diluent buffer
TBST

Incubation time
4C overnight

Incubation temperature:
RT

7) Secondary antibody:
Reacts against:
rabbit

Concentration or dilution
1:10,000

Diluent buffer
5% milk in TBST

Incubation time
1 hour

Incubation temperature:
room temp

Fluorochrome or enzyme conjugate:
HRP

8) Washing after primary and secondary antibodies:

Buffer
TBST

Number of washes
3

9)Detection method
ECL

10) How many times have you run this staining?
at least twice

Do you obtain the same results every time?
yes

What steps have you altered to try and optimize the use of this antibody?
change primary antibody concentration

Read More
Answer

Thank you for your reply with the requested protocol information.

I have review your protocol and I would like to make some suggestions to improve your results with this antibody.

You indicated in your protocol that you did not include any protease inhibitors in your lysis buffer. I would recommend including a cocktail of inhibitors to help prevent degradation of your samples. You may not be able to detect your protein of interest if you are observing multiple degradation products.

Additionally, have you tried another method of cell lysis? This protein may not be soluble in sample buffer alone and requires a different buffer for proper isolation.

Finally, we recommend testing the WB using BSA as a blocking agent (5%) for one hour at room temperature. Additionally, a 5% BSA solution should be used as diluent for the primary and secondary antibodies.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録