製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to VRK2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 260-360 of Human VRK2

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • 精製度
    Tissue culture supernatant
  • 特記事項(精製)
    Purified from TCS.
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Probable serine/threonine kinase.
  • 組織特異性
    Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis.
  • 配列類似性
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810003O05Rik antibody
    • AI447698 antibody
    • EC 2.7.11.1 antibody
    • Serine/threonine protein kinase VRK2 antibody
    • Serine/threonine-protein kinase VRK2 antibody
    • Vaccinia related kinase 2 antibody
    • Vaccinia virus B1R related kinase 2 antibody
    • Vaccinia-related kinase 2 antibody
    • VRK 2 antibody
    • VRK2 antibody
    • VRK2_HUMAN antibody
    see all

画像

  • Overlay histogram showing K562 cells stained with ab58052 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58052, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This image was generated using the ascites version of the product.

  • VRK2 antibody (ab58052) at 1ug/lane + K-562 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

  • ab58052 at 10 ug/ml staining VRK2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

    This image was generated using the ascites version of the product.

参考文献

ab58052 has not yet been referenced specifically in any publications.

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