保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PBS, pH 7.2
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精製度Protein G purified
Our Abpromise guarantee covers the use of ab58052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 58 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 10 µg/ml.|
機能Probable serine/threonine kinase.
組織特異性Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis.
配列類似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- 2810003O05Rik antibody
- AI447698 antibody
- EC 18.104.22.168 antibody
Overlay histogram showing K562 cells stained with ab58052 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58052, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
VRK2 antibody (ab58052) at 1ug/lane + K-562 cell lysate at 25ug/lane.
ab58052 at 10 ug/ml staining VRK2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
ab58052 has not yet been referenced specifically in any publications.