Application
Western blot
Loading amount
1000 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (Brain cancer cells (immunoprecipitated lysates))
Specification
Brain cancer cells (immunoprecipitated lysates)
Treatment
proteasome inhibitor MG-132
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Diluent: Milk 1% in TBS Tween 0.1%
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 110 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Immunoprecipitation of a 110 kDa protein from lysates of treated cells. Blot probed with ab7780.
Lane 1 : DMSO
Lane 2 : MG-132
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 22 2013